Changes in the VEGF-A concentration in the intraocular fluid of rats with alloxan model of diabetes at different durations of insulin therapy

Author: Boris Gagloev (Russian Federation)

Co-authors: Nadezhda Pozdeyeva, Issa Al Darraji

Purpose

To study the changes in the VEGF-A concentration in the intraocular fluid of rats with alloxan model of diabetes at different durations of insulin

Setting/Venue

The experiment was carried out on 212 outbred rats. In 197 rats, the alloxan model of diabetes was simulated by a single intraperitoneal injection of 100 mg/kg alloxan hydrate

Methods

7 days after administration of alloxan hydrate, the animals were divided into 3 groups. The main group consisted of animals with alloxan model of diabetes, which started single daily intraperitoneal injection of prolonged-acting insulin at a dose of 0.9 U/kg of body weight. The comparison group included animals with an alloxan model of diabetes, which did not receive specific therapy. The control group consisted of healthy animals that did not receive therapy. The experimental animals were withdrawn from the study 1 and 4 months after the start of insulin therapy. The concentration of VEGF-A was determined in 80—90 μl of intraocular fluid collected from both eyes of each animal

Results

At a 1-month period of insulin therapy, the VEGF-A concentration in the intraocular fluid in the study group (n = 17; 140 {136; 210} pg/ml) was statistically significantly higher than in comparison group (n = 20; 72 {58; 86} pg/ml; pm-u <0.0004), and in control group (n = 16; 76 {62.5; 88} pg/ml; pm-u = 0.0045). The comparison group did not have statistically significant differences when comparing the indicators of the control group (pm-u = 0.9979). At 4-months period of insulin therapy, the VEGF-A concentration in the intraocular fluid in the study group (n = 18) was 84.8 {61.1; 93.2} pg/ml, in comparison group (n = 16) - 66.4 {54.4; 73.75} pg/ml. The VEGF-A concentration in the intraocular fluid in the study group at the 4-month period of insulin therapy was statistically significantly lower than in the study group at the 1-month period of insulin therapy (pm-u <0.0044)

Conlusions

Insulin therapy during 1 month causes an increases the the concentration of VEGF-A in the intraocular fluid of rats with an alloxan model of diabetes, but after 4 months of insulin therapy, the VEGF-A concentration reduced to the initial values

Financial Disclosure

Authors do not have any financial interest in the subject matter. The study was funded by the Russian Foundation for Basic Research grant no. 18-315-00029.