Author: Christophe Chiquet (France)
Co-authors: Thibaud Mautuit, Stephen Hogg, Rachel Semecas, Nicolas Chateau, Emanuele Trucco, Vincent Daien
To compare retinal vascular diameter measurements taken from standard fundus images and adaptive optics (AO) images.
Prospective comparative study.
We analyzed retinal images of 20 healthy participants with 45-degree funduscopic color photographs (CR-2 Canon fundus camera, Canon™) and adaptive optics (AO) fundus images (rtx1 camera, Imagine Eyes®). Diameters were measured using three software applications: the VAMPIRE® (Vessel Assessment and Measurement Platform for Images of the REtina) Annotation Tool, IVAN (Interactive Vessel ANalyzer) for funduscopic color photographs, and AO_Detect_Artery™ for AO images.
For the arterial diameters, the mean difference between AO_Detect_Artery™ and IVAN measurements was 9.1 µm (-27.4–9.2 µm, p=0.005) with a significant correlation between measurements (r=0.79). The mean difference between the AO_Detect_Artery™ and VAMPIRE™ Annotation Tool measurements was 3.8 µm (-34.4–26.8 µm, p=0.16) with a weak correlation between measurements (r=0.12). For the venous diameters, the mean difference between the AO_Detect_Artery™ and IVAN measurements was 3.9 µm (-40.9–41.9 µm, p=0.35) with a strong correlation between measurements (r=0.83). The mean difference between the AO_Detect_Artery™ and VAMPIRE™ Annotation Tool measurements was 0.4 µm (-17.44–25.3 µm, p=0.91), and the correlation was moderate (r=0.41).
Taking AO imaging as a reference, we found that the VAMPIRE Annotation Tool, an entirely manual software, provides accurate measurements of the arterial and venular diameters, but the correlation is weak. By contrast, IVAN, a semi-automatic software tool, has slightly higher differences in measurements compared with AO imaging, but the correlation is stronger.
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