Author: Ayça Küpeli Çınar (Turkey)
Co-authors: Sadık Altan Özal, Rıza Serttaş, Abdulkadir Can Çınar, Suat Erdoğan
Purpose
Proliferative vitreoretinopathy (PVR) is a disease that commonly develops after retinal detachment surgery and causes an important threat to vision. The main characteristic of PVR is the migration, adhesion and epithelial-mesenchymal transition (EMT) of retinal pigment epithelial cells (RPE). There is currently no pharmacological agent effective in preventing the development of PVR or treating the disease. Eupatilin is a naturally occurring flavone that has the potential to inhibit cell proliferation and EMT of different cells. However, its efficacy on cell proliferation and migration caused by transforming growth factor-2 (TGF-β2) is unknown. In the present study, the potential impact of eupatilin on RPE cell proliferation and EMT was investigated.
Setting/Venue
Department of Ophthalmology, School of Medicine, Trakya University, Balkan Campus Edirne, Turkey Department of Medical Biology, School of Medicine, Trakya University, Balkan Campus, Edirne, Turkey
Methods
Serum starved human RPE cells (ARPE-19) were treated with 10 ng/ml TGF-β2 alone or co-treated with 25 μM eupatilin for 48 h. The mRNA and protein expression was evaluated by quantitative real-time PCR and Western blotting, respectively. Apoptosis and cell cycle progression were assessed by image-based cytometry. The effect of treatment on cell migration was evaluated by the wound healing assay.
Results
Eupatilin inhibited TGF-β2-induced RPE cell proliferation via regulating the cell cycle and inducing apoptosis. TGF-β2 upregulated mRNA expression of mesenchymal markers fibronectin and vimentin was significantly downregulated by eupatilin, while the epithelial markers E-cadherin and occludin expression was upregulated. Eupatilin administration significantly suppressed TGF-β2 encouraged cell migration through downregulating the expression of transcription factors Twist, Snail and ZEB1 induced by TGF-β2. Furthermore, eupatilin significantly inhibited the expression of MMP-1, -7 and -9, and suppressed NF-κB signaling.
Conlusions
These results suggest that eupatilin could inhibit the proliferation and transformation into fibroblast-like cells of RPE cells; thus eupatilin may be a potential therapeutic value in treating PVR.
Financial Disclosure
none
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