Author: Emine Kilic (Netherlands)
Co-authors: Daniel de Bruyn, Caroline van Rij, Nicole Naus, Dion Paridaens, Annelies de Klein, Erwin Brosens
Purpose
In uveal melanoma eye-sparing treatment options are being used increasingly. This poses a new challenge: without tissue, molecular analyses cannot be performed, and when therapeutic options arise early detection of metastases will be beneficial. To reduce prognostic uncertainty and detect metastases at an earlier stage, we aim to discover novel ways for prognostication without ocular biopsies. Minimally-invasive prognostication by capturing circulating tumor cells (CTCs) from peripheral blood of uveal melanoma patients. Especially in patients undergoing radiotherapy and for early detection of metastases.
Setting/Venue
The research presented was performed at the Erasmus MC, departments of Clinical Genetics, Ophthalmology and Radiotherapy Rotterdam The Netherlands in collaboration the Rotterdam Eye Hospital, Rotterdam, The Netherlands.
Methods
Nanobead-based immunocapturing of CTCs was performed using magnetic activated cell sorting (MACS). Multiple (uveal) melanoma bead-bound antibodies for capturing and markers for staining of CTCs were used. Blood was drawn at time of diagnosis and at different time points during stereotactic radiation treatment (SRT) and CTCs were isolated. Subsequently, plasma of patients with localized and metastatic disease was isolated and CTCs were retrieved. Slides were scanned and analyzed using Zeiss LSM700 microscope and software.
Results
Blood of patients undergoing stereotactic radiation treatment, metastatic patients and patients with localized disease was analyzed. We were able to quantify circulating tumor cells in 80% of irradiated patients and observed a difference in CTC yield for different days of irradiation. In patients with metastatic disease more CTCs were captured compared to those with localized disease.
Conlusions
CTCs can be captured in UM-patients and more CTCs are seen in patients receiving radiation treatment and when metastases arise. Thus, when no tumor tissue is available, CTC capturing could provide a novel way for minimally invasive prognostication.
Financial Disclosure
none
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